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ASSESSMENT OF THE SKIN SENSITISATION POTENTIAL IN THE MOUSE USING THE LOCAL LYMPH NODE ASSAY (LLNA) COUNTING CELL METHOD

OECD 429

PRINCIPLE OF THE TEST

The basic principle underlying the LLNA is that sensitizers induce proliferation of lymphocytes in the lymph nodes draining the site of test substance application. This proliferation is proportional to the dose and to the potency of the applied allergen and provides a simple means of obtaining a quantitative measurement of sensitization. Proliferation is measured by comparing the mean proliferation in each test group to the mean proliferation in the vehicle treated control (VC) group. The ratio of the mean proliferation in each treated group to that in the concurrent VC group, termed the Stimulation Index (SI), is determined, and should be ≥3 before classification of the test substance as a potential skin sensitizer is warranted.

In accordance with the regulations, the assessment of lymphocytes proliferation may be done without radioactive labelling to measure cell proliferation.

The method described here is based on the use of cell counting (by cell counter) to measure cell proliferation. The references of the international publications are presented in the last part of this protocol. This endpoint for assessment of the number of proliferating cells fully meets the performance standards requirement described in Annex 1 of the OECD No.429.

Price

€€

Turnaround

4 weeks

€ : < 1500 € €€ : between 1500 and 4000 € €€€ : between 4000 and 20000 € €€€€ : > 20000 €
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