OECD 427


For many substances, the exposure occurs transcutaneously that is to say through the skin.

The dermal absorption study in vivo provides elements necessary to extrapolate oral studies when safety assessments following dermal exposure. And, the in vivo method allows the determination of the penetration of the test substance through the skin in the entire organism. The test substance, preferably radiolabelled, is applied for a fixed period, on the shaved skin of the animal to one or more appropriate dose levels in the form of a preparation of representative use.

The rat is the species most commonly used. At least four animals of one sex should be used for each test preparation and each scheduled period. A known amount of the test preparation is applied. This amount should normally mimic potential human exposure. A reference exposure period (typically 6 or 24 hours) should be used, depending on the possible duration of human exposure.

Signs of toxicity and abnormal reactions were observed at intervals for the whole duration of the study.

This study includes analyzes of concentrations. regular detailed observations, as well as euthanasia at one time and blood samples for analysis.



18 weeks

€ : < 1500 € €€ : between 1500 and 4000 € €€€ : between 4000 and 20000 € €€€€ : > 20000 €
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