SKIN CORROSION : Transcutaneous Electrical Resistance (TER)

OECD 430


The test material is applied for up to 24 hours to the epidermal surfaces of skin discs in a twocompartment test system in which the skin discs function as the separation between the compartments.

The skin discs are taken from humanely killed rats aged 28-30 days. Corrosive materials are identified by their ability to produce a loss of normal stratum corneum integrity and barrier function, which is measured as a reduction in the TER below a threshold level . For rat TER, a cut-off value of 5kg has been selected based on extensive data for a wide range of chemicals where the vast majority of values were either clearly well above (often > 10 kΩ), or well below (often < 3 kΩ) this value . Generally, materials that are non-corrosive in animals but are irritating or non-irritating do not reduce the TER below this cut-off value. Furthermore, use of other skin preparations or other equipment may alter the cut-off value, necessitating further validation.

A dye-binding step is incorporated into the test procedure for confirmation testing of positive results in the TER including values around 5 kΩ. The dye-binding step determines if the increase in ionic permeability is due to physical destruction of the stratum corneum.

OCDE 430 TER P1040225



4 weeks

€ : < 1500 € €€ : between 1500 and 4000 € €€€ : between 4000 and 20000 € €€€€ : > 20000 €
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